부메뉴
자료실
Enantioseparation of Efavirenz
신고
인쇄
스크랩
Advances in Analytical Chemistry 2013, 3(3): 29-33
DOI: 10.5923/j.aac.20130303.02
Efavirenz은 AS-H로 키랄 분석이 가능합니다. 상세 내용은 첨부의 문헌을 통해 확인할 수 있습니다.
Enantioseparation of Efavirenz by Ultra Performance Liquid Chromatography
Surendra Dutt Sharma, Gaurav Singh*
Abstract A rapid isocratic chiral UPLC method has been developed for the separation of R-Efavirenz from S- Efavirenz. Good resolution viz. Rs> 3.0 between R- and S- forms of Efavirenz was achieved by NP-UPLC using chiral column Chiracel OD-H(250 mm x 4.6 mm) 5 μm. The mobile phase was n-Hexane and Isopropyl alcohol (IPA) in 90:10 ratio (v/v). Column temperature was 30°C and flow rate was kept 1.0 mL min-1.The elution was monitored by photodiode arraydetector at λ-254 nm. The calibration curve showed excellent linearity over concentration range 0.249-375 μg mL-1. This method was further used to determine the amount of R-Efavirenz in pure and active pharmaceutical ingredient of S-Efavirenz and is capable to quantitate and detect R-Efavirenz to the levels of 0.249 μgmL-1 and 0.075 μg mL-1 respectively. The average recovery range ofR- Efavirenzwas 97-104 %.
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